GSDMD Knockout cell line (HTR-8/SVneo)

location： Home > Products > KO Cell Lines > GSDMD Knockout cell line (HTR-8/SVneo)

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

GSDMD Knockout cell line (HTR-8/SVneo) Order

Catalog#

YKO-H257

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

GSDMD knockout cell line (HTR-8/SVneo)

Morphology

Endothelial cells, adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

GSDMD

Gene ID

79792

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

Target region

Knockout region: Exon 2 (based on transcript GSDMD-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HTR-8/SVneo			STR Info (Cell bank) Cell Line:HTR-8/SVneo
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	12		12	12
D13S317	9	12		9	12
D7S820	12	12		12	12
D16S539	11	13		13	13
VWA	13	18		13	18
TH01	6	9.3		6	9.3
AMEL	X	X		X	X
TPOX	8	8		8	8
CSF1PO	12	12		12	12

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GSDMD gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HTR-8/SVneo cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GSDMD gene knockout GSDMD knockout cell line (HTR-8/SVneo) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Related Popular Products

Wild-type Cell Lines

Human Trophoblast Cell Line(HTR-8/SVneo)
More details Order

Catalog#: YC-C050

Size: 1*10^6

Instruction:

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit
More details Order

Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250

Size: ~50 tests;~250 tests;~250 tests

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
More details Order

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

KO Cell Lines

GSDMD Knockout cell line (Hela)
inquiry More details

Organism: Human

Gene Name: GSDMD

Gene ID: 79792

Size: 1*10^6

Catalog#: YKO-XN89046

YKO-XN89046(Hela)
GSDMD Knockout cell line (HCT 116)
inquiry More details

Organism: Human

Gene Name: GSDMD

Gene ID: 79792

Size: 1*10^6

Catalog#: YKO-XN89045

YKO-XN89045(HCT 116)
GSDMD Knockout cell line (HEK293)
inquiry More details

Organism: Human

Gene Name: GSDMD

Gene ID: 79792

Size: 1*10^6

Catalog#: YKO-XN89044

YKO-XN89044(HEK293)

Related Popular Products

Wild-type Cell Lines

Human Trophoblast Cell Line(HTR-8/SVneo)

Price (USD): -

Catalog#: YC-C050

Size: 1*10^6

Instruction:

inquiry More details

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit

Price (USD): -;-;-

Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250

Size: ~50 tests;~250 tests;~250 tests

Instruction:

inquiry More details

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

Price (USD): -;-

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

inquiry More details

KO Cell Lines

GSDMD Knockout cell line (Hela)

Organism: Human

Gene Name: GSDMD

Gene ID: 79792

Size: 1*10^6

Catalog#: YKO-XN89046

inquiry More details
GSDMD Knockout cell line (HCT 116)

Organism: Human

Gene Name: GSDMD

Gene ID: 79792

Size: 1*10^6

Catalog#: YKO-XN89045

inquiry More details
GSDMD Knockout cell line (HEK293)

Organism: Human

Gene Name: GSDMD

Gene ID: 79792

Size: 1*10^6

Catalog#: YKO-XN89044

inquiry More details

✖

Phone

✖

Contact us✖

* Name：

Institution：

* Project Description：

* How would you like us to contact you?

* E-mail：

Phone：

WhatsAPP：

FaceBook Messenger：

Our Solution Specialists will contact you within 2 business days. Of course, you can also tell us when you would like to be contacted:

Select：

GSDMD Knockout cell line (HTR-8/SVneo)

Subscribe Us

GSDMD Knockout cell line (HTR-8/SVneo) Order

Detailed Product Information

Instruction

Related Popular Products

Wild-type Cell Lines

Human Trophoblast Cell Line(HTR-8/SVneo) More details Order

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit More details Order

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium More details Order

KO Cell Lines

GSDMD Knockout cell line (Hela) inquiry More details

GSDMD Knockout cell line (HCT 116) inquiry More details

GSDMD Knockout cell line (HEK293) inquiry More details

Related Popular Products

Wild-type Cell Lines

Human Trophoblast Cell Line(HTR-8/SVneo)

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

KO Cell Lines

GSDMD Knockout cell line (Hela)

GSDMD Knockout cell line (HCT 116)

GSDMD Knockout cell line (HEK293)

Gene Editing Services

EZ-editor™ Products

Contact us✖

Human Trophoblast Cell Line(HTR-8/SVneo)
More details Order

High-performance Mouse Genotyping Kit
More details Order

EZ-cryo™ Cell Freezing Medium
More details Order

GSDMD Knockout cell line (Hela)
inquiry More details

GSDMD Knockout cell line (HCT 116)
inquiry More details

GSDMD Knockout cell line (HEK293)
inquiry More details